Breeding for Wolf Sable puppies
Here are the different allele combinations needed in order to produce a puppy that is visually (phenotype) wolf sable :
MC1R : E/e or E/E (allows expression of dark/black pigment)
Brown : B/B or B/b (no dilution of the black pigment to brown.
Dilute : D/D or D/d (no dilution of the coat color to blue, lavender, etc.)
Dominant Black : N/N (allows expression of the agouti gene)
Agouti : aw/at or aw/a or aw/aw
ADDITIONAL NOTES :
A dog that is ay/ay or ay/aw or ay/at or ay/a will appear cream sable.
A dog that is aw/aw or aw/at or aw/a will appear wolf sable.
A dog that is at/at or at/a will appear black and tan.
A dog that is a/a will appear black.